Turkey acrosin. I. Isolation, purification, and partial characterization1
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چکیده
منابع مشابه
I-Induction, purification and partial characterization
In sea bass (males and non-vitcllogcnic females) estradiol (E2) treatment caused the appcarnnce in the bloodstream of a new protein that was identified as the vitellogenin (VTC) moleculc for this teleost spccies. The molecular weight of the sca bass VTG (445 kDa) and its amino acid composition was similar to that of other teleosts. VTC was purified by doublcstep chromatography and then used to ...
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Homogenates prepared from bovine heart muscle contain two distinct forms of malic enzyme (malate:NADP+ oxidoreductase (decarboxylating), EC 1.1.1.40), which can be easily separated by chromatography on DEAE-cellulose. A partial purflcation of the two enzymes has been accomplished by means of DEAE-cellulose chromatography, ammonium sulfate fractionation, and chromatography on CMSephadex. The iso...
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Acrosin is a proteolytic enzyme used by sperm to digest a path through the zona pellucida of the ovum. In ejaculated sperm it is inactivated by a proteinase inhibitor from seminal plasma that also inhibits trypsin. This inhibitor is removed or inactivated during the residence in the female reproductive tract as a part of the capacitation process. The boar acrosin-inhibitor complex was partially...
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An antibody was raised against a protein isolated from the cytoplasm of mesothelioma cells. It was subsequently used in an immunoperoxidase procedure on formalin fixed, paraffin embedded tissue sections. A representative sample of benign and malignant tumours from all the systems of the human body was examined. All the tumours derived from coelomic surfaces (mesotheliomas of pleura, peritoneum,...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1988
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod38.3.645